Derive one set of transform parameters for a panel — derive_logicle

Pooled across up to max_samples files so that every sample in a panel is transformed on the same scale. See the note at the top of this file for why that matters more than fitting each file well.

Usage

derive_logicle_pooled(
  reads,
  sids,
  markers = NULL,
  m = 4.5,
  a = 0,
  max_samples = 8L,
  max_events = 50000L,
  seed = 42L
)

Arguments

reads: list of read_fcs_resolved() results
sids: sample ids belonging to one panel
markers: marker names to parameterise
m, a: logicle display parameters
max_samples: files pooled
max_events: events drawn per file
seed: RNG seed; the stream is restored on exit

Value

named list of per-marker logicle parameters