QC heatmap for –absolute-counts: one tile per sample x population

WHAT: log10(cells/uL) as tile colour, one row per matched sample, one column per population, ordered by group where available. Blank tiles are values genuinely absent from the source file for that sample x population, not zero.

Usage

fig_absolute_counts_qc(
  ac,
  outfile,
  group_of = NULL,
  dpi = 200,
  colors = fcs_colors()
)

Arguments

ac

The ac.

outfile

Path to write the figure to.

group_of

Named character vector mapping sample_id to group label.

dpi

Resolution in dots per inch. May be reduced automatically to respect the raster ceiling; see safe_ggsave(). Default 200.

colors

Named list of colours; defaults to the package palette. See fcs_colors(). Default fcs_colors().

Details

WHY THIS COMES BEFORE THE GROUP-COMPARISON FIGURE (absolute_counts.png): this is externally supplied data this pipeline did not measure – a transcription slip, a stray order-of-magnitude error, or a sample that silently failed to match (see the NOTE lines the loader prints) will not look anomalous in a single boxplot the way it jumps out in a full grid. Controls and QC-failed samples are labelled rather than dropped, on the same principle fig_gating_qc() uses: a QC figure that hides the excluded rows cannot be used to catch a problem with them.