Where each population's uncertainty comes from

Stacked contribution per term, median across samples, in percentage points. The terms are the markers the population's definition reads, plus the CD45 parent gate, which enters every population at once because it sets the denominator.

Usage

fig_uncertainty_budget(budget, outfile, dpi = 200, colors = fcs_colors())

Arguments

budget

the budget element of run_gate_uncertainty()

outfile

Path to write the figure to.

dpi

Resolution in dots per inch. Default 200.

colors

Named list of colours; defaults to the package palette. Default fcs_colors().

Details

WHY IT IS WORTH A FIGURE OF ITS OWN. It says which gate to fix. A population whose budget is dominated by one marker has one threshold worth inspecting in gating_qc.png; a cohort whose budgets are dominated by the parent term has a CD45 gate problem that no amount of attention to the downstream markers will improve. Operator studies of manual gating find the same concentration at the first gate, which is the one place this figure and that literature can be read against each other.